BIOLOGY 65 – MICROBIOLOGY Spring 2008 – REVISED 2-26-08

INSTRUCTOR:      Jerry Hodge, Guy Van Cleave & Helen Yost

          OFFICE:    Redbud 7             Office Hours:  M 10-11,T 1:30-2:30, W 2-3, Th 1:30-3:30 

           PHONE:    Office 588-5158;   Lab Phone: 588-5157           email:  hodgej@yosemite.edu 

              TEXT:    Microbiology  by Tortora, Funke and Case (ninth edition)

LAB MANUAL:     Microbiology in Practice  by Lois Beishir (sixth edition)          

Most of the material on exams will be discussed in lecture.  Review sheets will be available for each exam.  Almost all of the test questions will be designed from these review sheets.   Much of the assigned reading is for background information.  Plan on reading the appropriate material before each lecture.  GET TO KNOW YOUR TEXT!!

 

SUPPLEMENT:    Six Modern Plagues and How We Are Causing Them by Mark Jerome Walters'

 

Supplemental Instruction: Patricia Horsthuis & Elizabeth Rousseau 8-9:30am; Th, 4-5pm T/Th in Seq 10

 

Maintain a copy of all work until final grades have been determined!!

GRADING:  There will be a TOTAL of 800 points in the class: 600 in Lecture and 200 in Lab.

                              A = 90% or above:        minimum 720 points

                              B = 80% or above:        minimum 640 points

                              C = 70% or above:        minimum 560 points

                              D = 55% or above:        minimum 440 points

 

      LECTURE:     Four Lecture Exams (make up policy*) worth          100 points each       400   points

                           Comprehensive Lecture Final Exam                                                      200   points     

                                                                                                                        600 LECTURE POINTS

 

      LABORATORY:                                                                                                          

                  Two Lab Exams:  Mid-term & Final                                    60 points each      120   points                 

                  Laboratory Skills:                                                                                           48   points

                     1)   Streak Plate of Mixed Culture:                                   3   points

                     2)   Fungi, 2 genera, slide cultures,  yeast wet mount:     10   points

                     3)   Gram Stain:                                                              5   points

                     4)   Endospore Stain:                                                     5   points

                     5)   Acid-fast Stain:                                                        5   points

                     6)   Negative Stain: (4 points if no spirochete is found)    5   points

                     7)   Unknown gram +/-;  turn in report:                            15   points

 

                  Reports (Data Sheets & Conclusions)  Due 2 weeks after the results are in:      32   points     

                     1)   Microscope/ Wet mount microbes                              2           points

                     2)   Anaerobic Culture Report:                                         8   points

                     3)   Recombination/Transformation Report:                      7   points

                     4)   Chemical Control Agents Report:                               7   points

                     5)   Nose & Throat Culture Report:                                   8   points

                    

                     .                                                                                                           200 LAB POINTS

 

*A make up exam if taken after the scheduled exam will consist of essay questions. Absences on the day of an exam will only be excused for college recognized circumstances, and only if the instructor is notified via email or voice mail prior to the scheduled time and day of the exam.  Written documentation (e.g., doctor’s notes, funeral notices, court papers, police report) is the optimal way to explain the circumstances regarding the missed exam.  Waking up late, or making personal travel plans that coincide with an exam date are not considered extreme circumstances and will not be considered for make up exams.  When in doubt, check with the instructor.  In addition, only one missed exam is allowed per semester.  If more than one exam is missed, even if excused, the student will not be allowed to make it up. There are no make up exams for missed lab practicals.

 

 

 

The following schedule only lists the days you are expected to begin an experiment.  Since most of our laboratory exercises require two or more periods to complete, it will be your responsibility to coordinate the work or several exercises at the same time.  When no assignment is listed on the schedule, it does not mean there is no lab work to be done.  Self preparation, in advance of the scheduled laboratory exercise, is also your responsibility.  The philosophy of the class is that the STUDENT DOES THE LEARNING.  YOU WILL BE PREPARED BEFORE YOU START ANY LAB WORK.  You will be expected to be on time to receive instructions on procedures and safety.  Irregular attendance in lab will lead to an automatic drop by the instructor.

BIOLOGY 65--MICROBIOLOGY LAB--TENTATIVE SCHEDULE    Spring 2008

Wk     Day   Date     LAB EXERCISE (M=Module in Lab Manual)

1.      Tu      1/8        Lab Introduction;  Lab safety rules; M1 Balance

         Th      1/10      M2, 3  Prepare, sterilize media (DEMO)

 

2.      Tu      1/15      M4  The Microscope

         Th      1/17      M5  Wet Mount; Termite symbionts; Bring Pond Water (optional)

 

         3.      Tu         1/22   M6, 7  Ubiquitous microbes: Bring interesting food sample; Aseptic technique, Media; Select for halophilic Archaea: Bring Rock Salt Selective, Enriched & Differential Media

         Th      1/24      M8, 9  Aseptic technique; Dilution theory (M42)

 

4.      Tu      1/29      M42  BRING MILK SAMPLE (raw or pasteurized)

         Th      1/31      M11,12, 13, 42 Evaluate milk; streak plates for isolation

 

5.      Tu      2/5        M21, 22 Simple stain; Procaryote & Eucaryote

         Th      2/7        M23  Gram stain

 

6.      Tu      2/12      M25  Endospore stain; Negative stain

         Th      2/14      M27  Acid-fast stain

 

7.      Tu      2/19      M28  Anaerobic cultures:  (continue with stains)

         Th      2/21      M16  Squash mounts DEMO; Set up fungi slide cultures & yeast wet mount

 

8.      Tu      2/26      DNA  technology – Transformation: blue-white screening in X-gal medium

         Th      2/28      Evaluate transformation; Evaluate slide cultures of fungi,

 

9.      Tu      3/4        Video: “When Wonder Drugs Don’t Work”; MRSA Detection

         Th      3/6        M31 Effects of Chemical Control Agents, BRING SAMPLES  , Lab Mid-term Review

 

10.     Tu      3/11      MID-TERM LAB EXAM

         Th      3/13      Evaluate Chemicals, set up static versus cidal; Streak unknowns

        

11.     Tu      3/18      M33, 34, 36, 39  Bacterial metabolism; Inoculate knowns; pure culture of unknowns   

         Th      3/20      Evaluate knowns with IMViC Tests; Unknowns

 

12.     Tu      3/25      Unknowns…….

         Th      3/27      Unkowns continued; M 37 Litmus Milk

 

13.     Tu      4/1        Unknowns continued; M37, 40, 49, 50:Parasite DEMOS, TSIA, Mannitol Salt Agar

         Th      4/3        M41 Water Analysis; BRING SAMPLES; Unknowns continued

 

14.     Tu      4/8        Nose & Throat Culture discussion and set up; Evaluate Water (M55) 

         Th      4/10      Nose & Throat results (Gram Stains etc.) 

 

15.     Tu      4/15      Final Review                                                           

         Th      4/17      LAB FINAL: All lab reports due prior to the beginning of the lab exam

        

 

 

 

 

 

 

BIOLOGY 65--MICROBIOLOGY LECTURE—REVISED-- SCHEDULE

Spring 2008

                                                                                                                            9th ed. Tortora et. al.

Wk  Day/Date    TOPIC                                                                                          Chapter/pages                                                          

1.   Tu  1/8        Introduction; History                                                                     Ch. 1

      Th  1/10      Classification; Chemistry                                                              Ch. 2

 

2.   Tu  1/15      Macromolecules; Microscopy                                                       Ch. 3

      Th  1/17      Functional anatomy of Prokaryotes                                               Ch. 4

 

3.   Tu  1/22      Functional anatomy of Eukaryotes, Metabolism, Enzymes              Ch. 4,5

      Th  1/24      Enzyme Inhibition, Redox, Oxidative Phosphorylation                    Ch. 5

 

4.   Tu  1/29      Exam 1, (lecture: Nutritional Patterns)                                        

      Th  1/31      Carbohydrate catabolism; Chemiosmosis; Fermentation                 Ch. 5

 

5.   Tu  2/5        Environmental terms, growth, Culture Media                                   Ch. 6, 7         

      Th  2/7        Microbial Growth; Control                                                             Ch. 6, 7

 

6.   Tu  2/12      Physical and chemical methods of control                                     Ch 7,8           

      Th  2/14      DNA Structure                                                                              Ch 8             

 

7.   Tu 2/19      transcription, translation, Genetic code                                          Ch. 8

      Th  2/21      EXAM 2;  Mutations

 

8.   Tu  2/26      Regulation of genetic expression; lac operon                                Ch. 8

      Th 2/28      Genetic transfer and recombination in bacteria                               Ch. 8

 

9.   Tu  3/4        Transposons, DNA technology                                                     Ch. 9

      Th  3/6        DNA Technology, Classification; Fungi                                          Ch. 10,12

 

10.  Tu  3/11      Fungi; Protista                                                                             Ch. 12

      Th  3/13      Animal parasites; Bacterial viruses                                                Ch. 12, 13

     

11.  Tu 3/18      EXAM 3; Lysogeny                                                                      Ch. 13 

      Th  3/20      Specialized Transduction; Animal viruses                                       Ch. 13,

 

12.  Tu  3/25      Prions; Normal microbiota, Disease                                              Ch. 13, 14

      Th  3/27      Mechanisms of Pathogenicity, Toxins                                           Ch. 14,15

 

13.  Tu  4/1        Modes of transmission; Epidemiology; Defense                            Ch. 14,15, 16

      Th  4/3        Immunity, Food poisoning, infections                                            Ch. 17, pp. 745-770

 

14.  Tu 4/8        Sexually Transmitted Diseases                                                      Ch. 26

      Th  4/10      EXAM 4 + STD’s

 

15.  Tu  4/15      Biogeochemical Cycles                                                                pp 809-828

      Th  4/17      Drugs & Evolution of Drug Resistance                                          Ch. 20

 

16.  Tu  4/22      Comprensive Lecture FINAL EXAM – 11 AM Dogwood

                        Bring two machine graded answer sheets and a No. 2 pencil

 

 

It is the philosophy of Columbia College that academic dishonesty is a completely unacceptable mode of conduct and will not be tolerated in any form.  All persons involved in academic dishonesty will be disciplined in accordance with College regulations and procedures.  Discipline may include suspension or expulsion from College.  Scholastic dishonesty includes but is not limited to cheating, plagiarism, collusion, the submission for credit of any work or materials that are attributable in whole or in part to another person, taking an examination for another person, any act designed to give unfair advantage to a student or the attempt to commit such acts.  You can get into trouble for plagiarism – even if you do not intend to cheat – by failing to correctly indicate places where you are making use of the work of another.  It is your responsibility to familiarize yourself with the conventions of citation by which you indicate which ideas are not your own and how yoru reader can find those sources.  Read your handbook for more information on quoting and citing properly to avoid plagiarism.  If your still do not understand, ask your instructor.

 

Columbia College is on record as being committed to both the spirit and letter of federal equal opportunity legislation; reference Public Law 93112 – The Rehabiliation Act of 1973 as amended.  With the passage of new federal legislation entitled Americans With Disabilities Act – (ADA), pursuant to section 504 of The Rehabilitation Act, there is renewed focus on providing this population with the same opportunities enjoyed by all citizens.  As a faculty member, I am required by law to provide “reasonable accommodation” to students with disabilities, so as not to discriminate on the basis of that disability.  Student responsibility primarily rests with informing faculty at the beginning of the semester and in providing authorized documentation through designated administrative channels.  Contact the Chief Operations Officer at 588-5112.

 

February 4, 2008 is the last day to withdraw without a “W” showing on your permanent record.